Advances in Brief
TOMOGRAPHY, September 2015, Volume 1, Issue 1: 30-36
NeuO for Neuronal Labeling in Zebrafish
1Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore; 2Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences, National University of Singapore, Singapore; and 3Department of Chemistry and MedChem Program of Life Sciences Institute, National University of Singapore, Singapore
We report the application of our newly developed fluorescent probe 3-(benzylamino)-4,4-difluoro-5-(4-propyl-1H-1,2,3-triazol-1-yl)-8-(4-(2-hydroxyacetamido)phen-yl)-4-bora-3a,4a-diaza-s-indacene (NeuO) to label and image live neurons in zebrafish. Immersing zebrafish embryos in NeuO or injecting NeuO into zebrafish brain ventricles results in nontoxic in vivo neuronal labeling. We demonstrate the applicability of NeuO and envisage the potential of this compound as a rapid and simple labeling reagent for studying neuron development and degeneration.