Advances in Brief

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TOMOGRAPHY, September 2015, Volume 1, Issue 1: 30-36
DOI: 10.18383/j.tom.2015.00127

NeuO for Neuronal Labeling in Zebrafish

Chai Lean Teoh1, Jun Cheng Er2, Parag Mukherjee3, and Young-Tae Chang1,3

1Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore; 2Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences, National University of Singapore, Singapore; and 3Department of Chemistry and MedChem Program of Life Sciences Institute, National University of Singapore, Singapore

Abstract

We report the application of our newly developed fluorescent probe 3-(benzylamino)-4,4-difluoro-5-(4-propyl-1H-1,2,3-triazol-1-yl)-8-(4-(2-hydroxyacetamido)phen-yl)-4-bora-3a,4a-diaza-s-indacene (NeuO) to label and image live neurons in zebrafish. Immersing zebrafish embryos in NeuO or injecting NeuO into zebrafish brain ventricles results in nontoxic in vivo neuronal labeling. We demonstrate the applicability of NeuO and envisage the potential of this compound as a rapid and simple labeling reagent for studying neuron development and degeneration.

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