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TOMOGRAPHY, March 2016, Volume 2, Issue 1: 3-16
DOI: 10.18383/j.tom.2016.00121

Radioactive Nanomaterials for Multimodality Imaging

Daiqin Chen1, Casey A. Dougherty1, Dongzhi Yang1, Hongwei Wu1, Hao Hong1

1Department of Radiology, University of Michigan, Ann Arbor, Michigan;2Center for Molecular Imaging, University of Michigan, Ann Arbor, Michigan; and3Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michigan


Nuclear imaging techniques, primarily including positron emission tomography and single-photon emission computed tomography, can provide quantitative information for a biological event in vivo with ultrahigh sensitivity; however, the comparatively low spatial resolution is their major limitation in clinical application. With the convergence of nuclear imaging with other imaging modalities like computed tomography, magnetic resonance imaging, and optical imaging, the hybrid imaging platforms can overcome the limitations of each individual imaging technique. Possessing versatile chemical linking ability and good cargo-loading capacity, radioactive nanomaterials can serve as ideal imaging contrast agents. Here, we provide a brief overview about the current state-of-the-art applications of radioactive nanomaterials in multimodality imaging. We present strategies for incorporation of radioisotope(s) into nanomaterials with the applications of radioactive nanomaterials in multimodal imaging. Advantages and limitations of radioactive nanomaterials for multimodal imaging applications are discussed. Finally, a future perspective of possible radioactive nanomaterial utilization is presented for improving diagnosis and patient management in a variety of diseases.


Molecular imaging has become a powerful tool for diagnosis and staging of multiple diseases and longitudinal treatment response monitoring (13). Imaging techniques such as magnetic resonance imaging (MRI), computed tomography (CT), ultrasonography, optical imaging, and nuclear imaging are widely used in different clinical scenarios (4). However, each individual imaging modality has inherent drawbacks (5, 6); thus, obtaining precise diagnostic information could be hampered by the use of a single imaging modality (7). Combining the merits of multiple imaging methods can provide for improved functional/anatomical information to be obtained; thus, researchers are more often using multimodality imaging platforms for their synergistic readouts (8, 9).

Integration of nuclear imaging approaches with other imaging modalities (10, 11) is rapidly advancing, as nuclear imaging (eg, positron emission tomography [PET] and single-photon emission computed tomography [SPECT]) provides whole-body detection with unparalleled sensitivity, good tissue penetration, and quantitative capacity (12, 13), with extremely high clinical value (13, 14). However, PET and SPECT imaging suffer from poorer spatial resolution; thus, the integration of PET or SPECT with other imaging methods with high spatial resolution, such as CT (1518), and more recently MRI (19), provides synergistic opportunities for improved clinical diagnosis and overall patient care (11, 20). An interesting fact is that not many standalone PET scanners have been sold in the marketplace since the introduction of PET/CT in 2001 (8); therefore, the combination of PET and CT has become the “gold standard” for oncological imaging. With better soft tissue contrast and lower radiation dose than CT, MRI becomes a new attractive choice to integrate with PET (21), and this integration can help to compensate for the low molecular sensitivity/specificity of MRI (22, 23). Optical imaging (eg, fluorescence), on the other hand, is less costly and provides real-time intraoperative guidance after the disease location is pinpointed by PET (or SPECT) (24).

Contrast agents, which can enhance image conspicuity for lesion detection, are desirable for improving molecular imaging sensitivity and specificity. For example, gadolinium (Gd) compounds (typically T1-weighted) and iron oxide materials (typically T2-weighted) are commonly used MRI contrast agents (25, 26). Because PET and SPECT imaging rely on the detection of γ-photons (511 keV pair or spontaneous) emitted from radioactive isotopes (eg, 18F [t1/2 = 110 minutes], 64Cu [t1/2 = 12.7 hours], 89Zr [t1/2 = 78.4 hours], and 99mTc [t1/2 = 6 hours]) (1214), the administration of contrast agents is indispensable. For successful multimodal imaging, a contrast agent with reliable performance and detectability by each imaging modality will be preferred. To achieve this goal, nanomaterials are very promising contrast agent candidates (2729). The main advantages of the nanomaterials include the following facts:

  1. Some nanomaterials are inherent contrast agents, for example, iron oxide nanoparticles (IONPs), which have received approval by the U.S. Food and Drug Administration (FDA) as MRI contrast agents (30, 31).

  2. Most nanomaterials possess large surface areas, so they can accommodate numerous contrast agent molecules, thereby increasing local concentration and detection sensitivity (32).

  3. Different functional groups or active sites on nanomaterials enable them to be chemically linked to contrast agents or disease-targeting ligands (33).

  4. Some nanomaterials can respond to specific stimuli (eg, heat, light, or pH fluctuation) for on-demand release of payloads, which may improve the contrast in a given region of interest where the stimuli exist (34).

  5. Nanomaterials can show selective accumulation in some disease sites. The well-known example is that nanomaterials with suitable size and morphology can distribute preferably at the tumor site through an enhanced permeation and retention effect (27).

Hence, multimodality imaging agents based on nanomaterials have undergone continuous improvements by research investigators (29).

An overview of the current state-of-the-art applications for radioactive nanomaterials as multimodality imaging contrast agents is provided in Table 1. With the extensive availability of PET scanners in clinics and the higher sensitivity of PET than of SPECT, we will focus more on the radioactive nanomaterials applicable for PET-multimodality imaging while also providing a brief summary on nanomaterials useful for SPECT. With the rapid development of each imaging technique and nanotechnology, we foresee that radioactive nanomaterials will eventually be adopted as irreplaceable clinical tools in the near future.

Table 1.

Representative Radioactive Nanomaterials for Multimodality Imaging

Core Nanomaterials Physical Properties Radiolabel Incorporation Method Intrinsic Imaging Capacity Utilization Synthesis Cost Representative References
Inorganic nanomaterials
    IONPs Paramagnetic (T2 contrast, T1 contrast when size is small) External chelator, isotope absorption, covalent linkage (18F) MRI LN mapping, tumor detection $ (53, 5860)
    Gold Fluorescence, photoacoustic signal, SERS External chelator, radioactive precursor Fluorescence, PAI, CRET Tumor targeting, image-guided surgery $$ (44, 93, 95, 99)
    QD fluorescence External chelator Fluorescence, CRET LN mapping, tumor detection/surgery $ (79, 80, 89, 90)
    Silica Biocompatibility, ultrahigh cargo-loading capacity, biodegradability External chelator, Isotope absorption N/A LN mapping, tumor detection/surgery (for C-dots), image-guided drug delivery $ (86, 88)
    Carbon nanomaterials Photothermal, fluorescence, photoacoustic signal, Raman signal External chelator, Fluorescence Tumor detection $$ (fullerene can be $$$) (25, 112)
    UCNPs luminescent External chelator, radioactive precursor (doping) UCL LN mapping, tumor detection $$$ (83, 103)
    Mn-/Gd-containing nanomaterials Paramagnetic (T1 contrast) External chelator, radioactive precursor MRI Tumor targeting, $$ (72, 113)
Organic nanomaterials
    Liposome Biocompatibility, optimal pharmacokinetics External chelator, isotope absorption Fluorescence, MRI (intrinsic label) Tumor targeting $ (68, 115)
    Polymers Biocompatibility, versatile chemistry External chelator, isotope absorption Fluorescence, PET (intrinsic label) Tumor targeting, image-guided drug delivery $ (69, 114, 116)

i] Abbreviations: IONPs – iron oxide nanoparticles; MRI – magnetic resonance imaging; PAI – photoacoustic imaging; CRET – Cerenkov resonance energy transfer; LN – lymph node; UCNPs – upconversion nanoparticles; UCL – upconversion luminescence; PET – positron emission tomography; SERS – surface-enhanced Raman scattering; QD - quantum dots.

Radioactive Nanomaterial Production

According to the chemical compositions, nanomaterials are classified into organic and inorganic nanomaterials. Common examples of organic nanomaterials include liposomes and polymers and dendrimers (35), and chemical compositions from inorganic nanoparticle families include silica-, iron oxide-, gold-, and carbon-based nanomaterials (30, 3638). Nanomaterials from both categories are useful tools for PET- or SPECT-fused multimodal imaging. To produce radioactive nanomaterials for imaging applications, the following 4 approaches have been undertaken to incorporate radioisotopes:

  1. An exogenous coordination compound (named a “chelator”) is added to the nanomaterial for binding radioactive metal ions (39).

  2. Proton or neutron beams are used to bombard the given atoms inside the nanomaterials to create postsynthesis radiolabels (40).

  3. Radioactive precursors (or preradiolabeled building blocks) are used to form radioactive nanomaterials (41, 42).

  4. Isotope absorption or exchange is used for postsynthesis radiolabeling (43, 44).

Each isotope incorporation approach has its own advantages and limitations. The attachment of the radioactive metal ions via exogenous chelators is simple and efficient, and it can be achieved at a relatively low cost. However, the stability of the resulting radiolabels has been a significant concern for this method, as radiometals can potentially be released from the chelator by isotope transchelation, and chelators themselves can be dissociated from the nanomaterial via enzymatic interactions in vivo. Chemical instability can compromise accurate evaluation of the pharmacokinetic behavior of radioactive nanomaterials in vivo. Direct radiolabeling methods by proton/neutron bombardment can largely avoid the above concerns, but the high cost and complicated instrumentation hinders practical use (40). Although the radioactive precursor method can form highly stable radioactive nanomaterials for imaging applications, unfortunately, the high radiation exposure during the production procedures is a significant working hazard (45). The chelator-free postsynthetic radiolabeling approach is a recently emerging method with low production cost and simplicity, although the stability and production yield of the resulting radioactive nanomaterials requires further improvement, and its application is currently limited to only a few nanomaterials. For future development, an optimal production method for radioactive nanomaterials should have high yields, stable products, short reaction times, low radiation exposure, and be easily adaptable to most nanomaterials (45). Development of new production methods and improvements of current strategies will promote new applications of radioactive nanomaterials. The current review presents an overview of the nanomaterials used in the context of their applicable imaging modality, as shown in Scheme 1.

Scheme 1.

Schematic illustration of radioactive nanomaterials for multimodality imaging.


Multimodality Imaging with Radioactive Nanomaterials


The first instrument to combine PET and MRI was developed in 2008 (19). Currently, both functional and anatomical data can be simultaneously collected by a modern PET/MRI scanner (46). Integration of PET and MRI endows the system with both high resolution and high sensitivity; thus, precise localization of the radioactive signals can be visualized within the context of anatomical features. Although a significant technical challenge, MRI can now provide attenuation correction for PET with clinically acceptable accuracy compared with CT-based attenuation correction (4749). Because of the sensitivity differences between the 2 imaging modalities, dual-modality contrast agents must consider the need to maintain a relatively low concentration of PET contrast (usually within the nanomolar range) along with a relatively high concentration of MRI contrast agent needed for sufficient MRI detection. Therefore, radioactive nanomaterials used in PET/MRI applications should ideally contain a sufficiently high MRI contrast ability along with a sufficient dose of radioactivity for PET detection. As a standout example, IONPs coupled with different isotopes served as the core of many PET/MRI imaging nanoplatforms (30).


Since IONPs have been approved by the FDA as clinically usable contrast agents for MRI (commercial name ferumoxytol), radioactive IONPs serve as the most popular PET/MRI agents (50). Given the fact that benefits and limitations of radiolabeled IONPs as dual-modality SPECT/MRI and PET/MRI imaging probes have already been summarized elsewhere (51), here we will briefly provide recent examples of IONP applications. 89Zr (zirconium-89), a PET isotope with a decay half-life (78.4 hours), is well matched to the circulation half-lives of antibodies or nanomaterials; as such, it is considered clinically relevant and has been reported in significant research activities over the last decade (52). 89Zr-labeled ferumoxytol was recently used for PET/MRI mapping of tumor-drained lymph nodes (LNs) in mice, as LN invasion is both critical for cancer staging and important for treatment planning (53). 89Zr was attached to ferumoxytol via ultrastable coordination with desferrioxamine (DFO) (Figure 1A), and the modification of ferumoxytol core with 89Zr–DFO did not alter its physicochemical properties such as size, charge, and magnetic properties. 89Zr–DFO–ferumoxytol provided sensitive tomographic detection of the tumor-drained axillary LNs in prostate tumor-bearing mice with high resolution (Figure 1A). Compared with the commonly used agent (99mTc-radiocolloid) for LN mapping, 89Zr–DFO–ferumoxytol shortened the diagnosis time and decreased the radiation dose to the test subjects. The IONP-based platform has significant translational potential to improve preoperative planning for nodal resection and tumor staging. By coupling with different PET isotopes (eg, 64Cu, 124I, 72As, and 69Ge), successful LN mapping was also achieved with these radioactive IONPs (5457).

Figure 1.

The application of 89Zr-ferumoxytol for normal lymph nodes (LNs) and tumor-drained LNs (A). Top panel: the structure of 89Zr-ferumoxytol. Lower left panel: detection of normal axillary LNs by 89Zr-ferumoxytol in positron emission tomography (PET)/magnetic resonance imaging (MRI). Lower middle panel: detection of the tumor-drained LN in Hi-Myc mouse by 89Zr-ferumoxytol in PET/MRI. Down right upper panel: PET/MRI of prostate region showing that the drained LN is outside of the prostate organ (green circle). Down right lower panel: distant drained inguinal node is identified by 89Zr-ferumoxytol (red arrow). Reproduced with permission from Thorek et al (53). The application of 64Cu-labeled, MoS2/IONP hybrid nanomaterial for PET- and MRI-based tumor detection (B). The structure of 64Cu-labeled MoS2/IONP is shown along with PET and MRI results at 24-hour after injection. Significant tumor uptake was confirmed in PET (circle indicates the tumor location) with a “darkened” tumor area in the MRI. Reproduced with permission from Liu et al (62).


Aside from LN mapping, radioactive IONPs can also be used for in vivo cancer targeting. For example, arginine–glycine–aspartic (RGD, a potent ligand for integrin αvβ3) peptide-conjugated 64Cu-labeled IONPs could efficiently accumulate inside different types of tumors and give clear tumor delineation in both PET and MRI (5860). More recently, hybrid nanostructures of IONPs [eg, with aluminum hydroxide (labeled with 18F) (61) or MoS2 nanosheets (labeled with 64Cu; Figure 1B) (62)] were also prepared for cancer imaging and subsequent image-guided cancer therapies. IONPs-based PET/MRI agents still possess certain drawbacks. Because IONPs are mostly used as T2-weighted contrast agents (negative contrast), image interpretation can be relatively difficult. Another concern is the aggregation of IONPs in vivo, which can alter the local signal intensity from MRI. A recent study demonstrated that aggregated IONPs, instead of IONPs alone, could produce significant artifacts in magnetic resonance (MR)-derived attenuation correction maps from PET/MRI (63). To overcome these limitations, T1-weighted contrast agents, for example, Gd and manganese (Mn) complexes, may be more preferred.

Gadolinium-Containing Nanomaterials.

Gd-containing nanomaterials are attractive MRI probes as long as proper functionalization has been conducted to maintain material integrity and prevent leakage of Gd ions. As an image contrast platform, the applicability of Gd oxide nanoparticles in PET/MRI and therapeutic delivery has been recently reviewed (64).

Fullerene is also a well-known delivery vector of Gd (38). A PET/MRI probe based on 124I-labeled Gd3N@C80 fullerene derivative was developed, and potential cytotoxicity from Gd leakage was avoided by caging the Gd ions inside the fullerene structure (25). Not only can this biocompatible Gd3N@C80 be used as a T1-weighted MRI agent and PET probe, it can also serve as a “radical sponge” to ameliorate inflammatory responses. Hydroxyl and carboxylic groups on the surface of Gd3N@C80 are also useful, as they allow the capability of additional functionalization. Tumors inside the glioblastoma-bearing rats could be distinctly visualized by 124I-labeled Gd3N@C80 from both PET and MRI. Rare-earth nanomaterials are another category of suitable nanoplatform for PET/MRI applications (65). Among them, Eu3+-doped Gd vanadate (GdVO4:Eu) nanosheets that have been synthesized by a solvothermal reaction in 1 study and further modified by 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) for 64Cu labeling and Asp-Gly-Glu-Ala (DGEA) peptide for integrin α2β1 cellular targeting (66). Prominent accumulation of 64Cu-DOTA-GdVO4:Eu-DGEA in PC-3 tumors (integrin α2β1+) was confirmed by both PET and MRI (Figure 2A), and tumor uptake was primarily mediated by integrin α2β1 targeting. In an interesting study, a 64Cu-labeled hybrid nanomaterial based on gold, Gd, and IONP was used for dual T1- and T2-weighted MRI and PET to delineate tumors (67). The resultant hybrid heterotrimers showed high physiological stability and could induce simultaneous positive and negative contrast enhancements in MR images. PET imaging studies revealed that the hybrid heterostructures showed favorable tumor delineation in mice, consistent with the MRI findings.

Figure 2.

Application of 64Cu-labeled GdVO4: Eu nanosheets for targeted tumor imaging (A). The schematic structure of 64Cu-DOTA-GdVO4: Eu nanosheets is shown. PET and MRI images of PC-3 (EphB4+) tumor-bearing mice at 24-hours after injection are shown for 64Cu-DOTA-GdVO4: Eu nanosheets with or without conjugation of the Asp-Gly-Glu-Ala (DGEA) peptide. Reproduced with permission from Hu et al (66). Application of 64Cu-labeled MnO@HSA nanoparticles for MRI and PET imaging of tumors (B). Upper panel: magnetic resonance (MR) images on U87MG xenografts acquired at 0, 1, 4, and 24 hours after 64Cu-labeled MnO@HSA injection. Lower panel: PET images taken at 1, 4, and 24 hours after 64Cu-labeled MnO@HSA injection. Reproduced with permission from Huang et al (72).


There are rather limited reports available on Gd-containing organic nanomaterials as PET/MRI agents. One such example is Gd-containing liposome (68). In this study, Gd was introduced via diethylenetriaminepentaacetic acid coordination, and 89Zr was incorporated by adsorption on lipid membranes. Octreotide, a peptide targeting human somatostatin receptor subtype 2 (SSTR2), was also linked to the liposome complex. Clearly, higher accumulation and retention in SSTR2+ tumors (acquired from PET/MRI), when compared with SSTR2 tumors in the same animal, were strong evidence that these 89Zr/Gd-containing liposomes showed excellent tumor-targeting ability in vivo. More recently, a glucose-based polymeric dextran nanomaterial (named “nanobeacon” by the authors) was also developed to retain 89Zr and Gd in a chelator-free manner (69). These 89Zr-nanobeacons could detect sentinel LNs and allow the surveillance of drug release from nanobeacons via MRI, as the MR signal from Gd could be quenched by the loaded drug on the nanobeacons.

Manganese-Containing Nanomaterials.

The T1-shortening properties qualify manganese as an MRI contrast agent (70). However, its biological toxicity hampered the development of otherwise useful applications such as cancer imaging, cell tracking, and brain imaging (71). Unlike Gd, an effective chelating agent with satisfactory binding stability for manganese has unfortunately not yet been identified for in vivo applications. Manganese-containing nanomaterials with sufficient in vivo stability may grant new biomedical applications to manganese. Surprisingly, using manganese-containing nanomaterials for PET/MRI is a current underexplored niche in contrast agent imaging research.

To the best of our knowledge, only 1 existing report has used 64Cu-labeled human serum albumin (HSA)-coated MnO nanoparticles for PET/MRI imaging of glioblastoma (72). The coating of HSA can increase the solubility of MnO nanoparticles and their longitudinal R1 relaxivity. These 64Cu-labeled MnO@HSA nanoparticles showed good physiological properties and stability along with superior T1 contrast. Tumor accumulation from 64Cu-labeled MnO@HSA was confirmed by both PET and MRI (Figure 2B). There are numerous opportunities ahead for manganese-containing nanomaterials to be used in PET/MRI studies, as the production of 52Mn (t1/2 = 5.6 days) has been optimized for PET applications (73). For future development, radiolabeled, hollow MnO nanoparticles (with better water accessibility) and stimulus-responsive manganese-containing nanomaterials are anticipated to be useful for improving both contrast agent sensitivity and specificity for detection of specific stimuli (74).


PET/Fluorescence (Luminescence).

The combination of PET and fluorescence/luminescence provides opportunities for radioactive nanomaterials to be used for fluorescence-/luminescence-guided surgery after initial detection of the disease site(s) via PET. There are 3 categories of radioactive nanomaterials that are useful for PET/fluorescence. In the first category, the nanomaterial has intrinsic fluorescence (eg, quantum dots [QD], gold nanomaterials and upconversion nanoparticles [UCNP]), which can be used for PET/fluorescence after direct radiolabeling. The second category involves nanomaterials labeled with both a radioisotope and a fluorophore. Sometimes, the loaded drugs (eg, doxorubicin) on the nanomaterial can also serve as a fluorophore for imaging purposes (7577). A third category involves radioactive nanomaterials that can be detected by both PET and Cerenkov luminescence imaging (CLI) from the same radiolabel. CLI is an emerging optical imaging modality based on the detection of Cerenkov radiation induced by particles emitted by a radioisotope as they travel through biological samples with a velocity faster than the speed of light (78). The progress in these 3 categories will be the focus of this section.

Radiolabeled QDs are the most prevalent nanomaterials for PET/fluorescence. QDs with different radiolabels [eg, 64Cu (79, 80) or 18F (81)] have been used for PET/fluorescence imaging of tumor vasculature with consistent readouts from both PET and fluorescence imaging modalities. Rare-earth UCNP is another type of nanomaterial with unique intrinsic fluorescence. It can absorb low-energy photons and emit high-energy photons (upconversion luminescence [UCL]), resulting in a very optimal signal-to-background ratio for imaging (82). UCNPs are ideal building blocks for multimodal imaging probes. For example, 18F-labeled, cyclodextrin-coated UCNPs were used for cell labeling and in vivo LN imaging via UCL/PET (83). The good biocompatibility from UCNPs encourages their use as multimodal imaging probes, although more reliable instrumentation will likely be needed for applications in UCL imaging. Other candidates such as red fluorescence-emitting zinc oxide nanoparticles (84) can also be useful for PET/fluorescence.

Postsynthesis incorporation of both fluorophore and radioisotopes is the most frequently adopted technique to produce PET/fluorescence-suited nanomaterials. For example, fluorescence-mediated tomography and PET were used to simultaneously measure protease activity, macrophage content, and integrin expression in the tumor by using a biocompatible IONP with 18F and a near-infrared (NIR) fluorophore (NIRF) attachment (85). Good correlations were shown between fluorescence-mediated tomography and PET in probe concentration and spatial distribution of signals.

Silica-based nanomaterials are important for PET/fluorescence imaging, where ample attention has been devoted on mesoporous silica nanoparticles (MSNs) and ultrasmall silica-based Cornell dots (C-dots). MSNs conjugated with 64Cu, 800CW (an NIRF dye), and a monoclonal antibody were adopted for PET/NIRF imaging of the tumor vasculature in 1 study (86). Good tumor-targeting efficacy and specificity in breast tumor-bearing mice were achieved for this 64Cu-labeled MSN, validated by PET and fluorescence. C-dots are the first PET/fluorescence nanoprobes that entered the clinical stage testing. After conjugation with 124I, an NIRF fluorophore (Cy5), and RGD peptide, C-dots were used as an integrin-targeting platform for imaging of melanoma metastasis with improved SLN (sentinel lymph node) localization and retention (Figure 3A), target-to-background ratios, and fast clearance from the site of injection and the body (87). The specificity of this C-dots platform, when compared with that of 18F-FDG, for metastasis/inflammation discrimination, was also satisfactory in the setting of surgery and therapeutic intervention. Furthermore, these radiolabeled C-dots were also used in a first-in-human clinical trial for lesion detection, cancer staging, and treatment management of patients with metastatic melanoma (88). 124I-RGD-C-dots(Cy5) showed superior in vivo stability, reproducible pharmacokinetic signatures (renal excretion), good tolerance in patients, and sensitive detection of small metastatic lesions (Figure 3A).

Figure 3.

Application of 124I-labeled, arginine–glycine–aspartic (RGD)-conjugated Cornell dots (C-dots) for clinical cancer detection and near-infrared fluorophore (NIRF)-guided surgery (A). Biocompatible C-dots could delineate a small pituitary lesion in a patient with metastasis patient in PET/MRI. The same-structured C-dots were successfully used for NIRF-guided tumor-drained LN removal. Reproduced with permission from Bradbury et al and Phillips et al (87, 88). Structure and application of intrinsically radioactive 64Cu-QDs for PET and Cerenkov resonance energy transfer (CRET) imaging (B). CRET luminescence photon flux was in a linear correlation with incorporated radioactivity. Consistent tumor uptake in U87MG tumors was revealed by PET and CRET. Reproduced with permission from Guo et al (90).


As stated previously, CLI enables the use of widespread luminescence rodent imaging equipment (eg, IVIS Spectrum) to visualize many commonly used medical isotopes (78), including clinical diagnostic (eg, PET) and therapeutic radionuclides. Compared with conventional optical imaging agents, CLI enables the use of approved radiotracers and does not require an external light excitation source, which would result in its rapid translation to clinical applications combining PET imaging and CLI-guided surgery with PET tracers. An emerging concept is to produce self-illuminating imaging agents (Cerenkov luminescence from isotopes served as the excitation source—named Cerenkov resonance energy transfer [CRET]) without autofluorescence background interference. Currently, only a few self-illuminating probes were developed, based mainly on QDs (89, 90), and 64CuCl2 was used as a synthesis precursor. These 64Cu-doped QDs showed excellent radiochemical stability and potent tumor uptake (Figure 3B), and these were successfully applied as efficient imaging agents for PET/self-illuminating luminescence in vivo. Radioactive gold nanocluster (64Cu-doped AuNCs) was another strong competitor for CRET-based PET/NIRF imaging (44), in which AuNCs acted as the energy acceptor for NIR fluorescence. 64Cu-doped AuNCs showed efficient CRET–NIR and PET signals, better passive targeting to tumors, and lower toxicity than QD conjugates. Although these studies were conducted in a preclinical setting (mostly mouse studies), the successful clinical translation of CLI-nanomaterials can be expected in the future, which will catapult radioactive nanomaterials toward increasingly versatile applications (91).

Other PET/Optical Imaging.

Compared with fluorescence imaging, other optical imaging techniques, such as Raman imaging or photoacoustic imaging, can also provide opportunities for integration as hybrid imaging applications with PET. Since its discovery, Raman spectroscopy, based on the inelastic scattering of a photon, has proven to be a powerful analytical tool offering many advantages including excellent sensitivity to small structural and chemical changes, its ability to multiplex, and its resistance to both autofluorescence and photobleaching (92). Although both radiolabeled noble metal nanomaterials and carbon nanomaterials can be used for PET/Raman imaging, most of the time Raman imaging is only a safeguard to ensure that material distribution information collected from PET is accurate. For example, the organ distribution of 64Cu-labeled gold nanoparticles was evaluated in mice by PET and validated by ex vivo Raman imaging via surface-enhanced Raman scattering (93). Raman imaging of excised tissues correlated well with distribution data from PET in this study (Figure 4A). The benefit of fusing Raman images onto PET images is that this combination can provide simultaneous surveillance of different materials/substances (with distinct Raman emissions) with excellent sensitivity (PET and Raman).

Figure 4.

PET and ex vivo Raman imaging to evaluate the organ distribution of 64Cu-labeled gold nanoparticles (A). Consistent organ uptake was obtained by PET and Raman signals. Reproduced with permission from Zavaleta et al (93). 64Cu-labeled melanin nanoparticles were used for tumor detection via PET and photoacoustic imaging (PAI) (B). The schematic structure of melanin nanoparticles (MNPs) is provided along with examples of both PET and PAI images of tumor-bearing mice. Reproduced with permission from Yang et al (96).


Photoacoustic imaging (PAI), based on the photoacoustic effect, is another attractive optical imaging technique with nonionizing electromagnetic waves, good resolution and contrast, portable instrumentation, and the ability to partially quantify the signal. PAI has been applied to the imaging of cancer, neurological disorders, vasculature function, and gene expression, among others (94). An anisotropic branched gold nanomaterial (Au-tripods) with superior optical properties was developed for PET/PAI (95). A linear correlation between PAI signals and Au-tripods concentration was confirmed in vivo. Intravenous administration of 64Cu-labeled, RGD peptide-conjugated Au-tripods (RGD–Au-tripods) to U87MG tumor-bearing mice showed PAI contrast in tumors almost 3-fold higher than for the blocking group, and PAI results correlated well with corresponding PET images. Au-tripods showed adequate selectivity and sensitivity for tumors in PET/PAI. In another study, the intrinsic PA signals and strong chelating properties (eg, for 64Cu) of melanin nanoparticles (MNP) were exploited to construct a PET/MRI/PAI agent (96). With apoferritin conjugation for transferrin receptor 1 (TfR1) targeting, this MNP showed excellent stability and presented good tumor uptake and high tumor contrast in HT29 tumor (TfR1+), with significantly lower accumulation in HepG2 (TfR1; Figure 4B).

Multimodality Imaging

Multimodality imaging platforms that combine more than 2 different imaging modalities have come into research focus (97, 98). To achieve this, nanomaterials used are usually in a hybrid structure or a core/shell architecture to embrace more contrast capacity from different components (99101).

Cui et al proposed 2 core–shell nanomaterials for trimodal (MRI, PET/SPECT and optical) imaging based on the integration of IONP and UCNP (102). The nanoparticles are composed of core–shell Fe3O4@NaYF4 nanoparticles with different metal ions doped (Yb, Er, Tm, etc.). With the stabilization from polyethylene glycol, the obtained nanoparticles showed high transverse relaxivity (R2) (326 mM−1−1 at magnetic field of 3T), good radiolabel stability, and strong upconversion luminescence. LNs in live mice could be clearly visualized by using 18F-labeled Fe3O4@NaYF4 (Yb, Tm) nanoparticles in PET, MRI, and UCL. With a similar design, hybrid gold-IONP nanoparticles were made, in which IONPs worked as a T2 MRI contrast agent, and the gold component acted as a strong fluorescence emitter and functionalization site (modified with 1,4,7-triazacyclononane-1,4,7-trisacetic acid for 64Cu labeling) (99). Anti-EGFR (EGFR stands for epidermal growth factor receptor) affibody was also included to provide tumor-targeting capabilities. As expected, the gold-IONP platform gave very sharp tumor contrast in PET, MRI, and fluorescence imaging. More recently, another more dramatic example is the hexamodal imaging by porphyrin–phospholipids-coated UCNP (PoP-UCNP) (103). To more fully utilize the imaging capacity of this nanomaterial, the authors characterized it both in vitro and in vivo for imaging via fluorescence, upconversion, PET, CT, CLI, and PAI (Figure 5).

Figure 5.

Hexamodal imaging with radioactive nanomaterials. Schematic structure and transmission electronic microscopy (TEM) images of this porphyrin-/lipid-wrapped upconversion nanoparticles (UCNPs) (A). Imaging studies with material-filling tubing by upconversion luminescence (UCL), fluorescence, PAI, PET, computed tomography (CT), and Cerenkov luminescence imaging (CLI) (B). Signal intensity–tissue depth relationship was also examined. (Note: +/− det means cover or remove turkey breast over the tubing). In vivo LN mapping by these 6 imaging modalities (C). Photoacoustic (PA) images before and after the material injection are shown. Reproduced with permission from Rieffel et al (103).


SPECT-Related Multimodality Imaging

For the last few decades, SPECT is the leading nuclear imaging technique because of the extensive use of 99mTc (t[1/2] = 6 hours), which can be conveniently obtained from 99Mo/99mTc generators (104). It is more established, less expensive, and more widely available than PET. One of the major advantages of SPECT imaging is that it can be used for simultaneous imaging of different radionuclides via the energy identification of the gamma photons emitted (105), thereby enabling simultaneous visualization of parallel biological events, although such strategy is not frequently adopted. From a material point of view, the key differences between a PET- and a SPECT-applicable nanomaterial are the specific radioisotopes used. Because PET possesses certain superiority (eg, higher detection sensitivity, better spatial resolution, and better quantitative capacity) and has become increasingly popular in both preclinical and clinical settings, SPECT-applicable nanomaterials will not be discussed in detail in this paper. Similar to PET isotope-included nanomaterials, radioactive nanomaterials can be used for SPECT/MRI and SPECT/optical, and additional combinations are possible (106108).

SPECT/MRI can be extremely helpful in scrutinizing the in vivo kinetics of radioactive nanomaterials (20, 106, 109, 110). For example, in vivo metabolism of polyethylene glycol (PEG)-modified ultrasmall paramagnetic iron oxide nanoparticles (USPIO, 1 type of IONPs), after labeling by 99mTc, could be monitored by both SPECT and MRI (Figure 6A) (111). 99mTc-PEG-IONP possess a high R1 relaxivity and a low R2/R1 to serve as an attractive T1-weighted MRI contrast agent. IONP-combined multiwalled carbon nanotubes (MWCNTs) were also used for SPECT/MRI after being further radiolabeled with 99mTc (112). Mouse imaging studies showed that the T2 contrast ability of superparamagnetic iron oxide nanoparticle (SPION)-MWCNTs was comparable with that of the clinically approved MRI contrast agent, Endorem. Organ distribution of SPION-MWCNTs acquired from SPECT, along with ex vivo transmission electronic microscopy and histological assessment, confirmed the integrity of SPION-MWCNTs in organs. Moreover, Gd-containing nanomaterials were also important participants in the SPECT/MRI studies. For example, hybrid Gd oxide nanoparticles (obtained by encapsulating Gd2O3 cores within a polysiloxane shell), which carried fluorophore Cy5 and (111) In, were used in SPECT, fluorescence, and MRI to evaluate their metabolism (eg, renal clearance) in rodents (113). A clear correlation was observed between modalities.

Figure 6.

Schematic structure of 99mTc-labeled ultrasmall paramagnetic iron oxide nanoparticles (USPIOs) (an iron oxide nanoparticle [IONP]) and clarification of its organ distribution by SPECT and MRI. T1-weighted images showing the increase in signal from blood in the vessels and the heart. SPECT/CT demonstrated similar pharmacokinetic profile for the 99mTc-labeled USPIO. Reproduced with permission from Sandiford et al (111). Schematic structure of EphB4-targeting micelles and their applications in SPECT/NIRF imaging of EphB4+ and EphB4 tumors (B). The EphB4 specificity of these micelles was validated by these two imaging modalities. Reproduced with permission from Zhang et al (114).


Many radioactive nanomaterials are useful for SPECT/fluorescence imaging or SPECT-involved multimodality imaging. Polymeric micelles conjugated with an EphB4 (a receptor tyrosine kinases overexpressed in many tumors)-binding peptide TNYL-RAW, an NIRF fluorophore Cy7, and 111In was used for tumor imaging via SPECT and NIRF (114). PC-3M tumors (EphB4+) could be clearly visualized by both SPECT and NIRF tomography after intravenous administration of 111In-labeled TNYL-RAW-micelles (Figure 6B). EphB4 specificity was confirmed from tumor uptake in A549 tumors (EphB4) and blocking experiments. Fluorescence signal from the nanoparticles correlated with their radioactivity count and colocalized with the EphB4-expressing region from histology. Liposomes incorporated with fluorescence labels and Gd or 111In were investigated in optical, MRI, and SPECT imaging for their cellular uptake and organ distribution (115). The ability to tune the imaging properties and distribution of these liposomes allows for the future development of a flexible trimodal imaging agent. Other more recent progress includes optically tunable nanomaterials featuring a unique design, where a single PEG polymer surrounds a fluorophore- and radiometal-bearing peptide (116). These nanomaterials could be applied for intraoperative angiography, measurements of capillary permeability, and tumor visualization by SPECT, for potential patient stratification.

Summary and Future Perspectives

There are 2 critical composing elements for a radioactive nanomaterial, that is, the radioisotope and the nanomaterial. For ready availability of radioactive nanomaterials for multimodality imaging, suitable selection of both components should be synergistic. On the one hand, incorporation of radioisotope(s) bestows extra tracking/therapeutic ability to the nanomaterial, which cannot be acquired by loading of other cargos. On the other hand, the utilization of suitable nanomaterials may serve as an isotope carrier and enable some unconventional isotopes to be used in specific biomedical applications, which may otherwise be very difficult to achieve, such as radioactive arsenic (eg, 72As) (57, 117), gemanium-69 (69Ge) (56), or sodium-22 (22Na) (118). Different imaging “labels” can be integrated into a single nanoplatform for combining the strengths of different imaging modalities, which can synergistically improve the overall value of imaging in the context of either basic research or patient care. In addition, nanomaterials with appropriate functionalization can evade attack from the immune system and thus create prolonged imaging time (45). Moreover, because most nanomaterials have large surface areas, which result in superior cargo accommodating capacity, they can help to increase local imaging contrast in selected areas. In addition, loading of imaging labels (isotopes/fluorophores, etc.) in nanomaterials can cause alterations of the in vivo pharmacokinetics of the labels, which can be tunable for image optimization in most cases.

Each imaging modality has its own advantages and limits. For example, the high sensitivity and good quantitative capability provided by PET/SPECT accompanies their low spatial resolution (typical >1 mm). The inherent low sensitivity of MRI and penetration limitations from optical imaging calls for combining the strengths of different imaging modalities to synergistically improve the information content provided by imaging. When radioactive nanomaterials are used in multimodality imaging, their stability is one of the most crucial factors for detection reliability, accuracy, and safety. The concept of “stability” here has dual meanings—radiochemical stability and stability of the nanomaterial itself. To acquire reliable and comparable imaging results, all the cargo(s) (particularly the radioisotopes) should stay adequately stable within the nanomaterial structure during the in vivo application, as PET or SPECT identifies the location of radionuclides rather than nanomaterials. Alternate functionalization/engineering strategies can be applied to not only optimize the stability of radioactive nanomaterials but also to provide the possibility for conjugation of a diverse number of different biological and bioactive molecules including drugs, proteins, and targeting ligands (32).

Another major challenge for radioactive nanoparticles is in optimization of their effectiveness to target specific disease phenotypes (39). Significant reports on radioactive nanomaterials used passive targeting only based on the enhanced permeation and retention effect, which is relying on the size, shape, surface charge, and circulation half-life of the nanoparticles. Although this can be therapeutically efficacious in some cases, this is by no means optimal for an imaging/diagnostic purpose. For example, the prolonged circulation half-life from a nanomaterial is a double-edged sword—although it can lead to a higher level of passive targeting to the tumor, it also causes prolonged exposure of the normal organs to the drug/radioisotope, which can give rise to undesired systemic toxicity. Active targeting is an approach that can enhance the preferential nanomaterial accumulation at disease site(s) via coupling with ligands that have selectivity and affinity toward diseased cells or tissues, or by a provided external stimulus (eg, a magnetic field) on a target cell/tissue spatial location (119). We can expect that significantly more research effort will be devoted to produce nanomaterials with active targeting capacity to improve multimodal image contrast. More specifically for oncological imaging, we believe that targeting of markers on tumor neovasculature will be more efficient for radioactive nanomaterials, as the size of many materials hinders their extravasation into the surrounding tumor parenchyma (120).

The majority of radioactive nanomaterials discussed in this paper have a hydrodynamic size range of 10–200 nm, which can cause persistent accumulation in the mononuclear phagocyte system (eg, liver and spleen). To ensure that a long-term safety profile can be achieved, careful radiation dosimetry and toxicological evaluation for each radioactive nanomaterial should be accomplished (121). In the meantime, suitable biological properties should be engineered into the design of the nanomaterial (eg, size/surface charge/degradability adjustment for fast renal clearance) in an effort to tune the in vivo distribution pattern to allow for injected contrast agents to be cleared within a reasonable period to meet subsequent FDA approval (122).

In summary, radioactive nanomaterials that can integrate multiple contrast agents into 1 single platform are important to realize real-time multimodality imaging. As multimodality imaging probes, radioactive nanomaterials should be able to provide for improved diagnostic accuracy. Continued research into the development of radioactive nanomaterials for imaging applications is anticipated to lead to, for example, radiolabeled IONPs that will be useful in simultaneous PET/MRI for early cancer diagnosis and disease staging. There are numerous opportunities and underexplored areas in radioactive nanomaterial research (eg, manganese nanomaterials), which we believe will serve as indispensable diagnostic and therapeutic tools in future medical applications. Overall, it is fully anticipated that continued advances in nanomaterials research will significantly improve clinical care and have a significant and positive impact on enhancing patient outcomes in the years ahead.


[2] Abbreviations:


Iron oxide nanoparticles




upconversion luminescence


multi-walled carbon nanotubes


photoacoustic imaging


Cerenkov luminescence imaging


quantum dots


fluorescence-mediated tomography


mesoporous silica nanoparticles


Cerenkov resonance energy transfer


melanin nanoparticles


upconversion nanoparticles


lymph node


upconversion nanoparticle


positron emission tomography


U.S. Food and Drug Administration


This work is supported in part by the University of Michigan Department of Radiology, the Elsa U. Pardee Foundation, and the National Institutes of Health (NCI P01 CA085878).

Conflict of Interest: None reported.


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