Advances in Brief

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TOMOGRAPHY, March 2017, Volume 3, Issue 1: 9-15
DOI: 10.18383/j.tom.2017.00112

MRI-Guided Stereotactic Biopsy of Murine GBM for Spatiotemporal Molecular Genomic Assessment

Stefanie Galbán1,2, Wajd N. Al-Holou4, Hanxiao Wang1,2, Amanda R. Welton1,2, Kevin Heist1,2, Xin Kathy Hu4, Roeland GW Verhaak4, Yuan Zhu5, Carlos Espinoza1,2, Thomas L. Chenevert1,2, Ben A. Hoff1,2, Craig J. Galbán1,2, and Brian D. Ross1,2

1Center for Molecular Imaging, University of Michigan Medical School, Ann Arbor, Michigan; 2Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan; 3Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan; 4The University of Texas MD Anderson Cancer Center, Houston, Texas; and 5Children’s National Medical Center, Washington, DC

Abstract

Brain tumor biopsies that are routinely performed in clinical settings significantly aid in diagnosis and staging. The aim of this study is to develop and evaluate a methodological image-guided approach that would allow for routine sampling of glioma tissue from orthotopic mouse brain tumor models. A magnetic resonance imaging-guided biopsy method is presented to allow for spatially precise stereotaxic sampling of a murine glioma coupled with genome-scale technology to provide unbiased characterization of intra- and intertumoral clonal heterogeneity. Longitudinal and multiregional sampling of intracranial tumors allows for successful collection of tumor biopsy samples, thus allowing for a pathway-enrichment analysis and a transcriptional profiling of RNA sequencing data. Spatiotemporal gene expression pattern variations revealing genomic heterogeneity were found.

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